antibacterial activity assay escherichia coli  (ATCC)

Journal: RSC Advances

Article Title: A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

doi: 10.1039/d6ra01785a

Figure Lengend Snippet: Cartoon representation of E. coli 70S ribosome (PDB ID 4v7v), where small (30S) and large (50S) subunits are displayed with ribosomal proteins on the left. The investigated region, including the peptidyl transferase center on 23S rRNA, is indicated on 50S on the right. The native inhibitor Clindamycin (CLY) is shown in sticks. Mg 2+ ions in the crystal structure are depicted as magenta spheres.

Article Snippet: Among these candidates, only Mitoxantrone and Daunorubicin exhibited antibacterial activity against E. coli ATCC 10536 (Table S4–S6).

Techniques:

Journal: RSC Advances

Article Title: A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

doi: 10.1039/d6ra01785a

Figure Lengend Snippet: In vitro inhibition of protein synthesis by Clindamycin (control) and the drug candidates in the E. coli cell-free translation system. Error bars represent standard error of the mean. Any error bars that are not visible are smaller than the data symbols.

Article Snippet: Among these candidates, only Mitoxantrone and Daunorubicin exhibited antibacterial activity against E. coli ATCC 10536 (Table S4–S6).

Techniques: In Vitro, Inhibition, Control